ゲノム科学部門

部門 >｜遺伝子構造機能｜遺伝子情報制御｜発生・分化・再生｜病態生理｜ゲノム科学｜遺伝子治療｜トランスレーショナルリサーチ

研究概要

　2003年4月にヒトゲノム塩基配列の完全解読が達成され、その後モデル動物として重要なマウスやラットのゲノムの塩基配列も決定され、時代はまさにポストシーケンスの時代、機能ゲノム学の時代に突入しています。遺伝子の塩基配列が決定されても、ゲノム上のどの部分が本当の遺伝子であるかは、まだ完全には同定されていません。ましてすべての遺伝子の機能の解明は、まだまだ今後の課題であります。当部門では、DNAマイクロアレイなどを用いたゲノムワイドな解析と、遺伝学的なアプローチから疾患遺伝子の同定や病態解明を行っています。ゲノム科学部門の研究の一つの大きな柱は、脂肪細胞分化あるいは骨芽細胞分化時の転写制御ネットワークの解明ならびに、それらの分化におけるクロストークの解明です（図１）。これらの転写制御ネットワークを解明することにより、メタボリック症候群や骨粗鬆症などの生活習慣病の病態解明ならびに、これらの病態改善のための創薬標的の描出あるいは診断マーカーの開発を目指しています。また、脂肪細胞分化における鍵転写因子であり、糖代謝、脂質代謝において創薬標的として重要な核内転写因子Peroxisome Proliferator-Activated Receptor（PPAR）を中心に新たな創薬標的の発見を目指し、またこれらの転写因子が制御する遺伝子を中心とした病態解明に力を入れています。
ヒトの病態を解明するために、モデル生物のゲノム・トランスクリプトーム・プロテオーム・フェノームなどの大量の情報を効率よく解析し、比較ゲノム学を用いた解析からヒトの病態に迫る研究も積極的に展開することにより、基礎研究と臨床研究の橋渡しを目指しています。

図1

図2

スタッフ

■教授(部門長)	岡崎　康司
■助教	水野　洋介
■助手	八塚　由紀子
■特任研究員	伊関　大敬, 徳澤　佳美, 森山　陽介
■ポストドクター	恩田　隆卓
■大学院生（博士）	菅原　泉, 田丸　俊輔
■特別研究学生	上原　奈津美
■特任研究補助員	高梨　孝子, 清水　千春
■客員教授	須田　立雄
■客員准教授	豊島　秀男
■共同研究員	山口　浩

研究テーマ

モデル生物として線虫・マウスを用い、主としてメタボリックシンドローム・糖尿病・虚血性疾患関連遺伝子の同定やその治療に関与しうる分子の同定を行っています。臨床のグループと共同して家族性疾患の原因遺伝子同定や薬剤感受性遺伝子同定も行っています。
1)	生活習慣病・多因子疾患（メタボリックシンドローム・糖尿病・骨粗鬆症など）における遺伝子ネットワークの描出とその遺伝的素因の解明
	1-1)	多分化能を持つ間葉系細胞を用い、脂質代謝と骨代謝に関連する遺伝子の転写制御ならびにクロストークを見出すことにより、病態ネットワークを解明し治療に応用する（図２）。
	1-2)	線虫を用いた代謝アッセイ系を開発し，網羅的RNAiスクリーニングを行い，ヒトの生活習慣病関連遺伝子を探索する。
	1-3)	生活習慣病、多因子疾患の病態におけるncRNA（非コードRNA)やmiRNA（マイクロRNA)の意義の探索
2)	臨床診断・治療を視野に入れたトランスレーショナル・リサーチ疾患遺伝子同定，診断マーカー開発，テーラーメード医療および新規生理活性物質の探索
3)	バイオインフォマティクスを駆使した比較ゲノム研究
	核内転写因子による遺伝子発現制御領域をコンピュータ解析し，生物種間比較解析も合わせて，新規なターゲット遺伝子を解明する．

主要文献

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REVIEW
53)	Bono H, Okazaki Y, Study of Mouse Metabolic Pathways Using cDNA Microarrays, "Prespectives in Gene Expression", PerkinElmer Life Sciences, 103-131 (2003)

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