埼玉医科大学雑誌 第30巻 第2号 (2003年4月) 121-129頁 ◇論文(図表を含む全文)は，PDFファイルとなっています．
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A Novel Oligosaccharide on Bovine Peripheral Myelin Glycoprotein P0
Jianhong Yan, Kunio Kitamura, and Masahiko Nomura
Department of Physiology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan
Abstract: Sulfated oligosaccharides have found in glycoproteins in myelin in the PNS, such as P0 and PASII/PMP22. These glycoproteins are involved in the interaction of cell membranes and play important roles in the formation and maintenance of myelin sheaths. These functions are due in part to the oligosaccharides in their molecules. Recently, the structures of the oligosaccharides of bovine peripheral myelin P0 were reported, and some of these carry the HNK-1 carbohydrate epitope (SO4-3GlcAβ1-3Galβ1-4GlcNAc). In this paper, we identified a novel multisulfated oligosaccharide, MS2, using one-dimensional 1H-NMR, mass spectrometry, and chemical and enzymatic analyses. MS2, which contains a HNK-1 epitope, was from a single N-glycosylation site of P0 glycoprotein. The structure of the novel oligosaccharide was: Manα1-3Manα1-6[(3SO4)GlcAβ1-3(6SO4)Galβ1-4(6SO4)GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc. In addition to the 3-O-sulfate present in the HNK-1 epitope, the oligosaccharide contained a 6-O-sulfated N-acetylglucosamine residue and a 6-O-sulfated galactose residue. This implies that both Gal6-O-sulfotransferase, which has not been reported in the PNS, and GlcNAc 6-O-sulfotransferase are active in the PNS tissues.
Keywords: Myelin, Glycoprotein, P0, Sulfated oligosaccharide, HNK-1, sulfotransferase.
J Saitama Med School 2003; 30: 121-129
( Received January 15, 2003)