埼玉医科大学雑誌 第31巻 第2号 (2004年4月) 89-94頁 ◇論文(図表を含む全文)は,PDFファイルとなっています.
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マウス胎仔腎臓由来細胞による培養下でのrenal tubulogenesisにおける形態学的検討
永野 忠相
埼玉医科大学総合医療センター第4内科〔平成16年1月9日 受付〕
Morphological Study
of the Renal Tubulogenesis in the Organoid Culture by Fetal Mouse
Kidney-derived Cells
Tadasuke Nagano (Fourth Department of Internal Medicine, Saitama Medical
Center, Saitama Medical School, Kawagoe, Saitama 350-8550, Japan)
The organoid culture method using completely isolated cells from fetal
organ is a useful tool for studying organogenesis. Using this method,
we investigated the renal development, especially renal tubulogenesis,
by a combination of electronmicroscopy, lectin-histochemistry, and reverse
transcription linked polymerase chain reaction (RT-PCR). Completely isolated
cells from fetal mouse kidneys were seeded onto a membrane filter. After
7 days in culture, a dome-shaped cellular mass consisted of tubule-like
structures was formed. Some short microvilli and basement membrane were
observed. After 14 days in culture, microvilli became longer, and the
number of microvilli was increased. The tubule-like structure showed
a positive staining of lectins[Dolicos biflorus agglutinin (DBA) and
Wheat germ agglutinin (WGA)]. Expression of aquaporin-2 (AQP-2) could
be detected after 1 day in culture. When the culture periods were prolonged,
the expression of AQP-2 was not detectable. Based on the findings of
morphological features and lectin-histochemical staining patterns, we
concluded that the tubule-like structure was recognized as a primitive
collecting duct. Organoid culture method presented here is a useful tool
for studying the development of the kidney.
Keywords: kidney, culture, tubulogenesis, lectin, collecting duct, aquaporin
J Saitama Med School 2004;31:89-94
(Received January 9, 2004)
(C) 2004 The Medical Society of
Saitama Medical School
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