埼玉医科大学雑誌 第31巻 第2号 (2004年4月) 89-94頁 ◇論文(図表を含む全文)は,PDFファイルとなっています.

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原 著
マウス胎仔腎臓由来細胞による培養下でのrenal tubulogenesisにおける形態学的検討

永野 忠相
埼玉医科大学総合医療センター第4内科〔平成16年1月9日 受付〕


Morphological Study of the Renal Tubulogenesis in the Organoid Culture by Fetal Mouse Kidney-derived Cells
Tadasuke Nagano (Fourth Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, Kawagoe, Saitama 350-8550, Japan)

The organoid culture method using completely isolated cells from fetal organ is a useful tool for studying organogenesis. Using this method, we investigated the renal development, especially renal tubulogenesis, by a combination of electronmicroscopy, lectin-histochemistry, and reverse transcription linked polymerase chain reaction (RT-PCR). Completely isolated cells from fetal mouse kidneys were seeded onto a membrane filter. After 7 days in culture, a dome-shaped cellular mass consisted of tubule-like structures was formed. Some short microvilli and basement membrane were observed. After 14 days in culture, microvilli became longer, and the number of microvilli was increased. The tubule-like structure showed a positive staining of lectins[Dolicos biflorus agglutinin (DBA) and Wheat germ agglutinin (WGA)]. Expression of aquaporin-2 (AQP-2) could be detected after 1 day in culture. When the culture periods were prolonged, the expression of AQP-2 was not detectable. Based on the findings of morphological features and lectin-histochemical staining patterns, we concluded that the tubule-like structure was recognized as a primitive collecting duct. Organoid culture method presented here is a useful tool for studying the development of the kidney.
Keywords: kidney, culture, tubulogenesis, lectin, collecting duct, aquaporin
J Saitama Med School 2004;31:89-94
(Received January 9, 2004)


(C) 2004 The Medical Society of Saitama Medical School
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