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医学博士 乙第918号 平成16年3月19日 （埼玉医科大学）
Studies on the Stimulative Effect of 3,000 Volts Alternating Current on Granuloma Formation and Collagen Synthesis
Yoshioka Koga (Department of Anesthesiology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan)
Rats raised on a 6% protein diet (low-protein diet) for three weeks have a reduced ability to synthesize collagen, and because the levels of this diet-induced reduced collagen synthesis are comparable to those for normally aged rats, low-protein diet-fed rats can be used as a model for aged rats. In the present study, 6-week-old male Wistar rats were raised on a low-protein diet for three weeks, and the effects of applying 3,000 V of alternating current and supplementing the low-protein diet with Eucommia ulmoides leaves on collagen synthesis were determined. 1. By applying 3,000 V of alternating current to the rats on the low-protein diet, the weight of granulation tissue and levels of hydroxyproline increased in a time-dependent manner when compared with the control group. After six weeks of applying 3,000 V of alternating current, the weight of granulation tissue and levels of hydroxyproline increased significantly by 187 and 171%, respectively (p<0.05). 2. By applying 3,000 V of alternating current to rats on the low-protein diet supplemented with Eucommia ulmoides leaves (0.75%), the weight of granulation tissue and levels of hydroxyproline increased significantly within four weeks by 136 and 163%, respectively (p<0.05 and 0.001, respectively). The results of the present study suggest that adding Eucommia ulmoides leaves facilitates collagen synthesis and formation of granulation tissue in aged rats. The fact that levels of hydroxyproline in granulation tissue increased significantly suggested that reduced collagen synthesis in aged rats was activated. The formation of granulation tissue is indicative of the early stages of wound healing, and this formation facilitates wound healing.
Keywords: Aged rat, Eucommia ulmoides leaves, 3,000 V of alternating current, Collagen synthesis, Granuloma formation