埼玉医科大学雑誌 第31巻 第3号別頁 (2004年7月) T45-T50頁 ◇論文(図表を含む全文)は,PDFファイルとなっています.

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Thesis
大腸癌におけるorotate phosphoribosyltransferase発現に関する検討

高橋 威洋
埼玉医科大学消化器一般外科(II)部門
(指導:平山 廉三教授)


医学博士 甲第935号 平成16年4月23日 (埼玉医科大学)


Expression of orotate phosphoribosyltransferase in colorectal cancer
Takehiro Takahashi (Department of digestive and general surgery, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan)

Backgroud: Activation of 5-fluorouracil (5-FU) into nucleotides requires phosphorylation by orotate phosphoribosyltransferase (OPRT). In this study, we investigated the correlation between enzymatic activity and gene expression of OPRT in colorectal cancer tissues, and the association between OPRT gene expression and anti-tumor effect.
Materials and Methods: Enzymatic activity and gene expression of OPRT were measured by radioassay method and a real-time reverse transcriptional-polymerase chain reaction method, respectively, in 20 primary colorectal cancer tissues. In 37 patients treated with tegafur-uracil and leucovorin for metastatic colorectal cancer, OPRT gene expressions were analyzed by the same method.
Results: There was a positive correlation between enzymatic activity and gene expression of OPRT (r=0.788, P<0.0001). Responding tumors had statistically higher OPRT gene expression than nonresponding tumours (P=0.0008), and patients with a high OPRT mRNA expression suvived longer than those with a low OPRT mRNA expression (P=0.0019).
Conclusion: The expression of OPRT gene might be useful as predictive parameter for the efficacy of fluoropyrimidine-based chemotherapy.
Keywords: orotate phosphoribosyltransferase, colorectal cancer, 5-fluorouracil


(C) 2004 The Medical Society of Saitama Medical School
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