埼玉医科大学雑誌 第32巻 第1号別頁 (2005年1月) T15-T25頁 ◇論文(図表を含む全文)は，PDFファイルとなっています．
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医学博士 甲第932号 平成16年3月31日 （埼玉医科大学）
BACKGROUND: Galectin-1 belongs to the galectin family of lectins, and is expressed in a variety of tissues including placenta, liver, skeletal and smooth muscle. Galectin-1 is thought to be a versatile modulator of cell function, but the biological significance of galectin-1 in human placenta is unclear. The galectin family comprises 14 proteins on the basis of amino acid sequence. METHODS: I raised a novel monoclonal antibody, Gal3D11, against human galectin-1, and characterized the epitope of the antibody using immunoblotting, high performance liquid chromatography, and mass spectrometry. RESULTS: The epitope of Gal3D11 comprises amino acids 29-48 of the galectin-1 protein. In this study, I used Gal3D11 to clarify the expression of galectin-1 at various stages of placental development. I found that galectin-1 was expressed specifically and ubiquitously in placenta trophoblasts, especially in syncytiotrophoblasts, during the first, second, and third trimester. CONCLUSIONS: The widespread expression of galectin-1 in placenta suggests that this protein plays an important role during the development of the placenta. In addition, Gal3D11 would appear to be an indispensable monoclonal antibody with which to further study the function of galectin-1 in different tissues.