Sho Matsushita, M.D., Ph.D.

English abstract is at the bottom of this homepage

　

　
Last updated: December 16, 2024

松下　祥
私の研究テーマはここ

トップページに戻る

生涯の主な研究成果（キーワード：抗原提示、Ｔ細胞、HLA）：

  1. 隠ぺい自己抗原による自己免疫病の発症機序をインスリン自己免疫症候群をモデルとして初めて分子レベルで明らかにしました（＠熊本大、J. Exp. Med. 180: 873-883, 1994）。 医系免疫学（中外医学社、改訂１６版）ｐ８６０などを御覧ください。また、美白剤ロドデノール誘発性白斑も隠ぺい自己抗原による自己免疫病であることを明らかにしました（＠埼玉医大、Dermatology 232:44-49, 2016）。 インスリン自己免疫症候群とともに １遺伝要因（特定のHLA対立遺伝子）と１環境要因（還元剤やロドデノール）の相互作用による自己免疫病発症の典型例です。 これらは不適切な質・量の自己抗原提示現象であり、広義の「ネオ・セルフ」であるとも言えます。
  2. 抗原提示において、HLA-DRとDQが抗原提示細胞内の異なるMAPKを活性化して異なるモノカインを誘導し、DRによる提示ではTh1が、DQによる提示ではTh2が誘導されやすいことを証明しました。 HLA-DRを介したシグナルについてはB細胞やヒト活性化T細胞においても発見がありました（＠熊本大、J. Biol. Chem. 275: 34998-35005, 2000, J. Immunol. 166: 2202-2208, 2001他多数)。
  3. ドーパミン受容体（D2様R）アゴニストとアデノシン受容体（A2aR）アンタゴニストが抗原提示相に作用し、T細胞のIL-17/IL-8応答の抑制を介して好中球性炎症を抑制することを発見しました。 自己免疫病やCOVID-19などへの応用が期待されます（＠埼玉医大、J. Immunol.186:3745-3752, 2011他多数）。

    学歴と職歴：

    １９７５年 大分県立佐伯鶴城高等学校卒業
    １９８１年 九州大学医学部卒業、九大病院第一内科・臨床研修医 （柳瀬敏幸教授）
    １９８３年 同・研究生（東京医科歯科大学にて研究） （笹月健彦教授）
    １９８３年 九州大学生体防御医学研究所遺伝学部門・助手 （笹月健彦教授）
    １９８８年 Medical Biology Institute (La Jolla, CA, U.S.A.)・Division of Immunology・Post-Doctoral Fellow （Dr. David Katz）
    １９９０年 同・Assistant Member （副主任研究員）
    １９９２年 熊本大学大学院医学研究科免疫識別学講座・助教授 （西村泰治教授）
    ２００１年 埼玉医科大学免疫学講座（医動物学を改名）・教授
    ２０１５年 埼玉医科大学・副学長(研究・産学連携等担当)
    ２０２４年 埼玉医科大学・研究統合推進センター長 ・特任教授

    資格等：

    １９８１年 ECFMG Standard Certificate
    １９８１年 第７１回医師国家試験合格、医師免許交付
    １９８２年 VQE (Visa Qualifying Examination)合格
    １９８７年 医学博士（九州大学）

    所属学会：

    ・日本アレルギー学会(功労会員)
    ・日本免疫学会
    ・自己免疫研究会

    受賞歴：

    ・熊杏会（熊本大学医学部同窓会）研究奨励賞
    ・加藤記念研究助成賞
    ・興和生命科学振興財団研究助成賞
    ・アストラ喘息研究奨励賞
    ・内藤記念科学振興財団研究奨励賞
    ・大山健康財団研究奨励賞
    ・日本リウマチ財団研究奨励賞
    ・金原一郎記念医学医療振興財団基礎医学医療研究助成賞
    ・北陸製薬・アレルギー学術奨励賞
    ・佐川がん研究助成振興財団研究助成賞
    ・持田記念医学薬学振興財団研究奨励賞
    ・財団法人大阪癌研究会学術研究助成賞
    以下、埼玉医大着任後
    ・浦上食品・食文化振興財団研究助成賞(２００２年)
    ・東京生化学研究会研究助成賞(２００２年)
    ・日本化学工業協会長期自主研究助成賞(２００５年)
    ・赤枝医学研究財団研究助成賞(２００６年)

    現在の学内活動：
    ・医学部教員代表者会議（オブザーバー）
    ・特許等委員会（オブザーバー）
    ・RAセンター運営会議（オブザーバー）
    ・研究統合推進センター運営会議（議長）

    受託研究：「CONTENTS」内の 「受託研究」をご覧下さい。

    主な論文：

    　

    総数328編（in press含む）：原著148編、総説132編、著書46編、症例報告2編より抜粋

    このホームページの最終部分をご覧下さい。

    2007年以降の主な論文／著書：

    1. Wakui, M., Nakano, K. and Matsushita, S. Notch ligand mRNA levels of human APCs predict Th1/Th2-promoting activities. Biochem. Biophys. Res. Commun. 358:596-601, 2007.
    2. 中野和久、松下　祥：樹状細胞とT細胞の相互作用とTh1/Th2細胞の分化。臨床免疫・アレルギー科 47:458−463, 2007.
    3. Matsushita, S. and Higashi, T. Human Th17 cell clones and natural immune responses. Allergol. Int. 5:135-140, 2008.
    4. Higashi, T., Wakui, M., Nakano, K., Hashimoto, K.,Takagi, R., Tanaka, Y. and Matsushita, S. Evaluation of adjuvant activities using human antigen presenting cells in vitro. Allergol. Int. 57:219-222, 2008.
    5. Takagi, R., Higashi, T., Hashimoto, K., Nakano, K., Mizuno, Y., Okazaki, Y. and Matsushita, S. B-cell chemoattractant CXCL13 is preferentially expressed by human Th17 cell clones. J. Immunol. 181:186-189, 2008.
    6. Nakano, K., Higashi, T., Hashimoto, K., Takagi, R., Tanaka, Y. and Matsushita, S. Antagonizing dopamine D1-like receptor inhibits Th17 cell differentiation: Preventive and therapeutic effects on experimental autoimmune encephalomyelitis. Biochem. Biophys. Res. Commun. 373:286-291, 2008.
    7. Nakano, K., Higashi, T., Takagi, R., Hashimoto, K., Tanaka, Y. and Matsushita, S. Dopamine released by dendritic cells polarizes Th2 differentiation. Int. Immunol. 21:645-654, 2009.
    8. Hashimoto, K., Inoue, T., Higashi, T., Takei S., Katayama S., Takagi, R., Okada, H., Matsushita, S. Dopamine D1-like receptor antagonist, SCH23390, exhibits a preventive effect on diabetes mellitus that occurs naturally in NOD mice. Biochem. Biophys. Res. Commun. 383:460-463, 2009.
    9. Okada,H., Inoue T., Hashimoto K., Suzuki H., Matsushita S. D1-Like Receptor Antagonist Inhibits IL-17 Expression and Attenuates Crescent Formation in Nephrotoxic Serum Nephritis. Am. J. Nephrol. 30:274-279, 2009.
    10. 中野　和久、松下　祥、齋藤　和義、山岡　邦宏、田中　良哉：樹状細胞-T細胞間における免疫修飾物質としてのドパミンの機能と関節リウマチの病態形成における役割。日本臨床免疫学会会誌32:1-6, 2009.
    11. Higashi T, Hashimoto K, Takagi R, Mizuno Y, Okazaki Y, Tanaka Y and Matsushita S. Curdlan induces DC-mediated Th17 polarization via Jagged1 activation in human dendritic cells. Allergol. Int. 59:161-166, 2010.
    12. Nahoko Kato-Kogoe, Hideki Ohyama, Fusanori Nishimura, Michio Meguro, Sayuri Yoshizawa, Yuka Okada, Keiji Nakasho, Koji Yamanegi, Naoko Yamada, Masaki Hata, Takehiro Higashi, Nobuyuki Terada, and Sho Matsushita. Fibroblasts stimulated via HLA-II produce PGE 2 and regulate cytokine production from Th cells.Lab. Invest. 90:1747-1756, 2010.
    13. 松下　祥、高木理英：Th17細胞の産生ケモカインとその意義。臨床免疫・アレルギー科, 53:229-234, 2010.
    14. 東　丈裕、松下　祥：Th17細胞を分化させる諸要因。臨床免疫・アレルギー科, 53:407-412, 2010.
    15. Nakano, K., Yamaoka, K., Hanami, K., Saito, K., Sasaguri, Y., Yanagihara, N., Tanaka, S., Katsuki, I., Matsushita, S and Tanaka Y. Dopamine Induces IL-6_Dependent IL-17 Production via D1-Like Receptor on CD4 Naive T Cells and D1-Like Receptor Antagonist SCH-23390 Inhibits Cartilage Destruction in a Human Rheumatoid Arthritis/SCID Mouse Chimera Model. J. Immunol. 186:3745-3752, 2011.
    16. Nakashioya, H., Nakano, K., Watanabe, N., Miyasaka, N., Matsushita, S., Kohsaka, H. Therapeutic effect of D1-like dopamine receptor antagonist on collagen-induced arthritis of mice. Modern Rheumatol. 21:260-266, 2011.
    17. Nakagome, K., Imamura, M., Okada, H., Kawahata, K., Inoue, T., Hashimoto, K., Harada, H., Higashi, T., Takagi, R., Nakano, K., Hagiwara, K., Kanazawa, M., Dohi, M., Nagata, M., Matsushita, S. Dopamine D1-like receptor antagonist attenuates Th17-mediated immune response and OVA-Ag-induced neutrophilic airway inflammation. J. Immunol. 186:5975-5982, 2011.
    18. Matsushita, S., Takagi, R., Hashimoto, K., Higashi, T. Qualitative evaluation of adjuvant activities and its application to Th2/17 diseases. Int. Arch. Allergy Immunol. 155:2-5, 2011.
    19. Higashi, T., Shimojo, N., Suzuki, S., Nakaya, M., Takagi, R., Hashimoto, K., Nakagome, K., Nakamura, K., Kohno, Y. and Matsushita, S. Coenzyme A contained in mothers’ milk is associated with the potential to induce atopic dermatitis. Int. Immunol. 23:741-749, 2011.
    20. 松下　祥：アジュバント活性の多様性。漢方と免疫・アレルギー 24:138-165, 2011.
    21. Nakagome, K., Matsushita, S., Nagata, M. Neutrophilic inflammation in severe asthma. Int. Arch. Allergy Immunol. 158:96-102, 2012.
    22. 松下　祥：樹状細胞のドーパミン産生とＴｈ１７細胞の誘導。臨床免疫・アレルギー科58:165-170, 2012.
    23. Mori, Y., Kabashima, K., Fukamachi, S., Kuroda, E., J, Sakabe., Kobayashi, M., Nakajima, S., Nakano, K., Tanaka, Y., Matsushita, S., Nakamura, M., Tokura, Y. D1-like dopamine receptors antagonist inhibits cutaneous immunereactions mediated by Th2 and mast cells. J. Dermatol. Sci. 71:37-44, 2013.
    24. Takagi, R., Kawano, M., Nakagome, K., Hashimoto, K., Higashi, T., Ohbuchi, K., Kaneko, A. and Matsushita, S. Wogonin attenuates ovalbumin antigen-induced neutrophilic airway inflammation by inhibiting Th17 differentiation. Int. J. Inflamm., Vol. 2014, Article ID 571508, 8 pages.
      http://dx.doi.org/10.1155/2014/571508
    25. Rie Takagi, Masaaki Kawano and Sho Matsushita. Dopamine modulates Th17-mediated autoimmunity via dopamine receptors expressed on dendritic cells and naive CD4 T cells. Current Trends in Immunology. 15:9-17, 2014.
    26. Masaaki Kawano, Rie Takagi, Atsushi Kaneko and Sho Matsushita. Berberine is a dopamine D1-, D2-like receptor antagonist and ameliorates experimentally induced colitis by suppressing innate and adaptive immune responses. J. Neuroimmunol. 289:43-55, 2015.
    27. Rie Takagi, Masaaki Kawano, Koichiro Nakamura, Tetsuya Tsuchida and Sho Matsushita. T-cell responses to tyrosinase-derived self-peptides in patients with leukoderma induced by rhododendrol: Implications for immunotherapy targeting melanoma. Dermatology 232:44-49, 2016.
    28. Miyano, K., Matsushita, S., Tsuchida, T., and Nakamura, K. The inhibitory effect of a histamine 4 receptor antagonist on CCL17 and CCL22 production by monocyte-derived Langerhans cells in patients with atopic dermatitis. J. Dermatol. 43:1024-1029, 2016.
    29. 高木理英、松下祥：ロドデノール誘発性白班症は自己免疫病である。臨床皮膚科71:42-45, 2017.
    30. Yutaka Enomoto, Rie Takagi, Yutaka Naito, Tsuyoshi Kiniwa, Yasuhito Tanaka, Susumu Hamada-Tsutsumi, Masaaki Kawano, Sho Matsushita, Takahiro Ochiya, and Atsushi Miyajima. Identification of the novel 3’ UTR sequences of human IL-21 mRNA as potential targets of miRNAs. Scientific Reports7:7780, 2017.
    31. Itano, A., Kawano , M., Takagi, R., Tokuyama, K. and Matsushita, S. Signaling via FcεRI stimulates human immature dendritic cells to induce Th1 differentiation via IL-12: Novel evidence of a feedback loop of allergic responses mediated by dendritic cells. Current Trends in Immunology 18:57-65, 2017.
    32. Matsueda, H., Kawano, M., Takagi, R., Koshimizu, K. and Matsushita, S. Acetylcholine stored in dendritic cells plays a role in the differentiation of naive CD4+ T cells into a Th1 phenotype. Clin. Exp. Neuroimmunol. 9:115-123, 2018.
    33. Matsuyama, T., Kawano, M., Takagi, R., Nakagome, K., Chikamatsu, K., Matsushita, S. IL-8 produced by T cells is under the control of dopamine signaling. Clin. Exp. Neuroimmunol. 9:251-257, 2018.
    34. Nishizawa, K., Takagi, R., Kawano, M and Matsushita, S. Protein kinase C activation via serotonin receptor induces IL-8 in antigen-presenting cells stimulated with diazinon. Current Topics in Toxicology 14:41-52, 2018.
    35. Kawano, M., Takagi, R.,Saika, K., Matsui, M and Matsushita, S. Dopamine regulates cytokine secretion during innate and adaptive immune responses. Int. Immunol. 30:591-606, 2018. DOI:10.1093/intimm/dxy057
    36. Kawano, M., Saika, K., Takagi, R., Matsui, M. and Matsushita, S. Tannic acid acts as an agonist of the dopamine D2L receptor, regulates immune responses, and ameliorates experimentally induced colitis in mice. Brain, Behavior, & Immunity-Health 5:100071, 2020.
    37. Kikue Saika, Masahiko Kato, Hideaki Sanada, Sho Matsushita, Masanori Matsui, Hiroshi Handa and Masaaki Kawano. Induction of adaptive immune responses against antigens incorporated within the capsid of simian virus 40. Journal of General Virology DOI 10.1099/jgv.0.001445
    38. Rie Takagi, Masaaki Kawano, Tsuyoshi Sato and Sho Matsushita. Tannic acid, a dopamine receptor agonist, ameliorates periodontitis, atopic dermatitis and psoriasis in animal models. Current Trends in Immunology 22:11-17, 2021.
    39. Tokano, M., Kawano, M., Takagi, R. and Matsushita, S. Istradefylline, an adenosine A2a receptor antagonist, ameliorates neutrophilic airway inflammation and psoriasis in mice. Clin. Exp. Neuroimmunol. 12:268-275, 2021.
    40. 松下　祥：胎児が免疫で排除されないのはなぜ？産科と婦人科89: 1169-1174, 2022.
    41. Mieko Tokano, Rie Takagi, Masaaki Kawano, Shigefumi Maesaki, Norihito Tarumoto and Sho Matsushita. Signaling via dopamine and adenosine receptors modulate viral peptide-specific and T-cell IL-8 response in COVID-19. Immunol. Med. 45:162-167, 2022.
      https://doi.org/10.1080/25785826.2022.2079369
    42. Mieko Tokano, Masaaki Kawano, Rie Takagi, and Sho Matsushita. Istradefylline, an adenosine A2a receptor antagonist, inhibits the CD4+T-cell hypersecretion of IL-17A and IL-8 in humans. Immunol. Med.45:244-250, 2022.
      https://doi.org/10.1080/25785826.2022.2094593
    43. Yuta Isozaki, Tsuyoshi Sato, Rie Takagi, Ko Ito, Michihiko Usui, Masaaki Kawano, Sho Matsushita. Ropinirole inhibits inflammatory cytokine production in gingival epithelial cells and suppresses alveolar bone loss in an experimental rat model of periodontitis. Experimental and Therapeutic Medicine 25: 78, 2022.
      DOI: 10.3892/etm.2022.11777
    44. Mieko Tokano, Sho Matsushita, Rie Takagi, Toshimasa Yamamoto, and Masaaki Kawano. Extracellular adenosine induces hypersecretion of IL-17A by T-helper 17 cells through the adenosine A2a receptor. Brain, Behavior, & Immunity - Health26:100544, 2022.
    45. Masaaki Kawano, Rie Takagi, Mieko Tokano, and Sho Matsushita. Adenosine induces IL-31 secretion by T-helper 2 cells: Implication for the effect of adenosine on atopic dermatitis and its therapeutic strategy. Biochem. Biophys. Res. Commun. 645:47-54, 2023.
    46. Tokano,M., Takagi, R., Matsushita, S. Modifications in immune response patterns induced by kynurenine and one-residue-substituted T cell epitopes in SARS-CoV-2-specific human T cells.COVID 4: 1676-1683
    47. Takeharu Watanabe, Masayuki Hayashi, Masaaki Arai, Sho Matsushita, Hiroshi Handa, Masaaki Kawano.Interferon-γ production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles. Heliyon inpress.

      48. Brief CV：


      DOB: 1956
      Business Address: Project Professor and Director, Center for Integrated Research Activities, Saitama Medical University, 38 Morohongo, Moroyama 350-0495, JAPAN
      e-mail: shomat@saitama-med.ac.jp

      1975-1981: Faculty of Medicine, Kyushu University (M.D.)
      1981-1983: Resident Internist, 1st Department of Internal Medicine, Kyushu University (Fukuoka, Japan)
      1983-1987: Assistant Professor, Medical Institute of Bioregulation, Kyushu University (Fukuoka, Japan)
      1987: Doctor of Medical Science (Ph.D.)
      1988-1989: Post-doctoral fellow, Division of Immunology, Medical Biology Institute (La Jolla, CA, USA)
      1990-1992: Assistant member, Division of Immunology, Medical Biology Institute (La Jolla, CA, USA)
      1992-2001(Aug.): Associate Professor, Division of Immunogenetics, Kumamoto University Graduate School of Medical Sciences (Kumamoto, Japan)
      2001(Sep.)-: Professor and Chair, Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University (Moroyama, Japan)
      2015(Apr.)-: Vice-President for Research, Saitama Medical University
      2024(Apr.)-: Project Professor and Director, Center for Integrated Research Activities

      　

      Research Projects：

      I was involved in an immunogenetic analysis of cedar pollinosis in Dr. Sasazuki's laboratory for my PhD degree. Then, I spent 5 years to study IgE regulation in La Jolla. After I came back to Japan in 1992, my work was focused on HLA-peptide-TCR interactions, and successfully proved “cryptic-self” in insulin autoimmune syndrome, as well as rhododendrol-induced vitiligo in humans.
      Recently, my research interest is focused on immunomodulation by neurotransmitters. Many neurotransmitters are expressed by immune cells, called immunotransmitters. Modulation of these activities can ameliorate autoimmune diseases and other neutrophilic inflammation, including allergy and infections, such as COVID-19.

      Representative publications (of 328publications, as of April 10, 2023)

      1. Masaaki Kawano, Rie Takagi, Mieko Tokano, and Sho Matsushita. Adenosine induces IL-31 secretion by T-helper 2 cells: Implication for the effect of adenosine on atopic dermatitis and its therapeutic strategy.
        Biochem. Biophys. Res. Commun. 645:47-54, 2023.
      2. Mieko Tokano, Sho Matsushita, Rie Takagi, Toshimasa Yamamoto, and Masaaki Kawano. Extracellular adenosine induces hypersecretion of IL-17A by T-helper 17 cells through the adenosine A2a receptor.
        Brain, Behavior, & Immunity - Health 26, 100544, 2022.
      3. Mieko Tokano, Rie Takagi, Masaaki Kawano, Shigefumi Maesaki, Norihito Tarumoto, Sho Matsushita. Signaling via dopamine and adenosine receptors modulate viral peptide-specific and T-cell IL-8 response in COVID-19.
        Immunol. Med. 45:162-167, 2022.
      4. Masaaki Kawano, Kikue Saika, K, Rie Takagi, Masanori Matsui and Sho Matsushita. Tannic acid acts as an agonist of the dopamine D2L receptor, regulates immune responses, and ameliorates experimentally induced colitis in mice.
        Brain, Behavior, & Immunity-Health 5: 100071, 2020.
      5. Masaaki Kawano, Rie Takai, Kikue Saika, Masanori Matsui and Sho Matsushita. Dopamine regulates cytokine secretion during innate and adaptive immune responses
        Int. Immunol.30:591-606, 2018.
      6. Yutaka Enomoto, Rie Takagi, Yutaka Naito, Tsuyoshi Kiniwa, Yasuhito Tanaka, Susumu Hamada-Tsutsumi, Masaaki Kawano, Sho Matsushita, Takahiro Ochiya, and Atsushi Miyajima. Identification of the novel 3’ UTR sequences of human IL-21 mRNA as potential targets of miRNAs
        Scientific Reports 7:7780, 2017.
      7. Rie Takagi, Masaaki Kawano, Koichiro Nakamura, Tetsuya Tsuchida and Sho Matsushita. T-cell responses to tyrosinase-derived self-peptides in patients with leukoderma induced by rhododendrol: Implications for immunotherapy targeting melanoma.
        Dermatology 232:44-49, 2016 .
      8. Masaaki Kawano, Rie Takagi, Atsushi Kaneko and Sho Matsushita. Berberine is a dopamine D1-, D2-like receptor antagonist and ameliorates experimentally induced colitis by suppressing innate and adaptive immune responses.
        J. Neuroimmunol. 289:43-55, 2015.
      9. Takagi, R., Kawano, M. and Matsushita, S. Dopamine modulates Th17-mediated autoimmunity via dopamine receptors expressed on dendritic cells and na夫e CD4 T cells.
        Current Trends in Immunology. 15:9-17, 2014.
      10. Nakagome, K., Matsushita, S. and Nagata, M. Neutrophilic inflammation in severe asthma.
        Int. Arch. Allergy Immunol.158:96-102, 2012.
      11. Higashi, T., Shimojo, N., Suzuki, S., Nakaya, M., Takagi, R., Hashimoto, K., Nakagome, K., Nakamura, K., Kohno, Y. and Matsushita, S. Coenzyme A contained in mothers’ milk is associated with the potential to induce atopic dermatitis.
        Int. Immunol. 23:741-749, 2011.
      12. Nakagome, K., Imamura, M., Okada, H., Kawahata, K., Inoue, T., Hashimoto, K., Harada, H., Higashi, T., Takagi, R., Nakano, K., Hagiwara, K., Kanazawa, M., Dohi, M., Nagata, M., Matsushita, S. Dopamine D1-like receptor antagonist attenuates Th17-mediated immune response and OVA-Ag-induced neutrophilic airway inflammation.
        J. Immunol. 186:5975-5982, 2011.
      13. Nakano, K., Yamaoka, K., Hanami, K., Saito, K., Sasaguri, Y., Yanagihara, N., Tanaka, S., Katsuki, I., Matsushita, S and Tanaka Y. Dopamine Induces IL-6_Dependent IL-17 Production via D1-Like Receptor on CD4 Naive T Cells and D1-Like Receptor Antagonist SCH-23390 Inhibits Cartilage Destruction in a Human Rheumatoid Arthritis/SCID Mouse Chimera Model.
        J. Immunol. 186:3745-3752, 2011.
      14. Hashimoto, K., Inoue, T., Higashi, T., Takei S., Katayama S., Takagi, R., Okada, H., Matsushita, S. Dopamine D1-like receptor antagonist, SCH23390, exhibits a preventive effect on diabetes mellitus that occurs naturally in NOD mice.
        Biochem. Biophys. Res. Commun. 383:460-463, 2009.
      15. Nakano, K., Higashi, T., Takagi, R., Hashimoto, K., Tanaka, Y. and Matsushita, S. Dopamine released by dendritic cells polarizes Th2 differentiation.
        Int. Immunol. 21:645-654, 2009.
      16. Nakano, K., Higashi, T., Hashimoto, K., Takagi, R., Tanaka, Y. and Matsushita, S. Antagonizing dopamine D1-like receptor inhibits Th17 cell differentiation: Preventive and therapeutic effects on experimental autoimmune encephalomyelitis.
        Biochem. Biophys. Res. Commun.373:286-291, 2008.
      17. Takagi, R., Higashi, T., Hashimoto, K., Nakano, K., Mizuno, Y., Okazaki, Y. and Matsushita, S. B-cell chemoattractant CXCL13 is preferentially expressed by human Th17 cell clones.
        J. Immunol. 181:186-189, 2008.
      18. Wakui, M., Nakano, K. and Matsushita, S. Notch ligand mRNA levels of human APCs predict Th1/Th2-promoting activities.
        Biochem. Biophys. Res. Commun. 358:596-601, 2007.
      19. Matsushita, S., Ohyama, H., Kudo, H., Tabata, H. and Matsuoka, T. HLA-mediated signaling via HLA-peptide-TCR complex determines immune responses of antigen-presenting cells.
        Current Topics in Peptide & Protein Research. 6:1-20, 2004.
      20. Matsushita, S., Tanaka, Y., Tabata, H., Matsuoka, T., Ohyama, H. and Nakashima, T. Combinatorial peptide library for the analysis of antigen recognition by T cells.
        Combinatorial Chemistry and High Throughput Screening 5:539-551, 2002.
      21. Kudo, H., Matsuoka, T., Mitsuya, H., Nishimura, Y ., Matsushita, S. Cross-linking HLA-DR molecules on Th1 cells induces anergy in association with increased level of cyclin-dependent kinase inhibitor p27Kip1
        Immunol. Lett. 81:149-155, 2002.
      22. Matsushita, S., Tanaka, Y., Matsuoka, T. and Nakashima, T. Clonal expansion of freshly isolated CD4T cells by randomized peptides and identification of peptide ligands using combinatorial peptide libraries.
        Eur. J. Immunol. 31:2395-2402, 2001.
      23. Matsuoka, T., Tabata, H. and Matsushita, S. Monocytes are differentially activated through HLA-DR, -DQ and -DP molecules via MAP kinases.
        J. Immunol. 166:2202-2208, 2001.
      24. Tabata, H., Matsuoka, T., Endo, F., Nishimura, Y. and Matsushita, S. Ligation of HLA-DR molecules on B cells induces enhanced expression of IgM heavy chain genes in association with Syk activation.
        J. Biol. Chem. 275: 34998-35005, 2000.
      25. Tanaka, Y., Ohyama, H., Ogawa, M., Nishimura, Y. and Matsushita, S. Identification of peptide superagonists for a self-K-ras-reactive CD4+ T cell clone using combinatorial peptide libraries and mass spectrometry.
        J. Immunol. 162:7155-7161, 1999.
      26. Matsushita, S., and Matsuoka,T. Peptide length-dependent TCR antagonism on class II HLA-restricted responses of PBMC and T-cell clones.
        Eur. J. Immunol. 29:431-436, 1999.
      27. Fujita, H., Senju, S., Yokomizo, H., Saya, H., Ogawa, M., Matsushita, S. and Nishimura, Y. Evidence for human CD4+ T cell clones specific to p53 cryptic self peptides and responding to p53 proteins of both wild and mutant forms.
        Eur. J. Immunol. 28:305-316, 1998.
      28. Matsushita, S., Kohsaka, H. and Nishimura, Y. Evidence for self- and non-self-peptide partial agonists that prolong clonal survival of mature T cells in vitro.
        J. Immunol. 158:5685-5691, 1997.
      29. Matsuoka, T., Kohrogi, H., Ando, M., Nishimura, Y. and Matsushita, S. Altered TCR ligands affect antigen-presenting cell responses: Upregulation of IL-12 by an analogue peptide.
        J. Immunol. 157:4837-4843, 1996.
      30. Kira, J-I, Kanai, T., Nishimura, Y., Yamasaki, K., Matsushita, S., Hasuo, K., Tobimatsu, S. and Kobayashi, T. Western versus Asian types of multiple sclerosis: two immunogenetically as well as clinically distinct disease entities.
        Annals Neurol. 40: 569-574, 1996.
      31. Matsushita, S., Nishi, T., Oiso, M., Yamaoka, K., Yone, K., Kanai, T. and Nishimura, Y. HLA-DQ-binding peptide motifs. I. Comparative binding analysis of type II collagen-derived peptides to DR and DQ molecules of rheumatoid arthritis-susceptible and non-susceptible haplotypes.
        Int. Immunol. 8:757-764, 1996.
      32. Chen, Y-Z., Matsushita, S. and Nishimura, Y. Response of a human T cell clone to a large panel of altered peptide ligands carrying single residue substitutions in an antigenic peptide: characterization and frequencies of TCR agonism and TCR antagonism with or without partial activation.
        J. Immunol. 157:3783-3790, 1996.
      33. Ikagawa, S., Matsushita, S., Chen, Y-Z., Ishikawa, T. and Nishimura, Y. Single amino acid substitutions on a Japanese cedar pollen allergen (Cry j I)-derived peptide induced qualitative changes in human T cell responses and T cell receptor antagonism.
        J. Allergy Clin. Immunol.97:53-67, 1996.
      34. Matsushita, S., Takahashi, K., Motoki, M., Komoriya, K., Ikagawa, S. and Nishimura, Y. Allele specificity of structural requirement for peptides bound to HLA-DRB1*0405 and -DRB1*0406 complexes: Implication for the HLA-associated susceptibility to methimazole-induced insulin autoimmune syndrome.
        J. Exp. Med. 180:873-883, 1994.
      35. Marcelletti, J.F., Matsushita, S. and Katz, D.H. B cell activator: Effects on B cell expression of CD23, proliferation and antibody secretion.
        J. Immunol. 148:3857-3863, 1992.
      36. Matsushita, S., Marcelletti, J.F., Katz, L.R. and Katz, D.H. Purification of murine suppressive factor of allergy into distinct CD23-modulating and IgE-suppressive proteins.
        Proc. Natl. Acad. Sci. USA 88:4718-4722, 1991.
      37. Hirayama, K., Matsushita, S., Kikuchi, I., Iuchi, M., Ohta, N. and Sasazuki, T. HLA-DQ is epistatic to HLA-DR in controlling the immune response to schistosomal antigen in humans.
        Nature 327:426-430, 1987.
      38. Matsushita, S., Muto, M., Suemura, M., Saito, Y. and Sasazuki, T. HLA-linked nonresponsiveness to cryptomeria japonica pollen antigen. I. nonresponsiveness is mediated by antigen-specific suppressor T cell.
        J. Immunol. 138:109-115, 1987.