• 教授　　池田　正明

    ・略歴：

    1983年　　岡山大学医学部卒業
    1987年　　岡山大学大学院（内科系）修了　医学博士
    1987年　　アリゾナ大学医学部薬理学　博士研究員
    1989年　　国立精神・神経センター・神経研究所　流動研究員
    1991年　　国立精神・神経センター・神経研究所　研究員
    1993年　　埼玉医科大学第一生理学　助手
    2000年　　埼玉医科大学第一生理学　講師
    2001年　　埼玉医科大学生理学　講師
    2001年　　埼玉医科大学ゲノム医学研究センター
    プロジェクト研究部門・分子時計プロジェクト・チームリーダー（兼坦）
    2006年　　埼玉医科大学医学部生理学　助教授
    2007年　　埼玉医科大学医学部生理学　准教授
    2009年　　埼玉医科大学医学部生理学　教授

    ・専門：
    神経科学、時間生物学

    ・研究テーマ：
    分子時計プロジェクトでは、現在、以下のような研究テーマを中心に取り組んでいます。

    1. 概日リズムの分子機構の解明
    -時計遺伝子BMAL1の機能解析を中心として-
    2. 概日リズムと恒常性の維持・代謝調節機構の解明
    3. 時計遺伝子と細胞増殖・腫瘍との接点の解明
    4. 転写の定量・イメージング技術の開発と応用

    業績　（埼玉医大着任以降）
    Oshima T, Niwa Y, Kuwata K, Srivastava A, Hyoda T, Tsuchiya Y, Kumagai M, Tsuyuguchi M, Tamaru T, Sugiyama A, Ono N, Zolboot N, Aikawa Y, Oishi S, Nonami, A, Arai F, Hagihara S, Yamaguchi J, Tama F, Kunisaki Y, Yagita K, Ikeda M, Kinoshita T, A Kay AS, Itami K, Hirota T: Cell-based screen identifies a new potent and highly selective CK2 inhibitor for modulation of circadian rhythms and cancer cell growth. Science Advances, 5(1), eaau9060, 2019

    Takenaka Y, Inoue I, Nakano T, Ikeda M, Kakinuma Y, Ikegami Y, Shimada A, Noda M:
    Evaluation of Teneligliptin Effects on Transcriptional Activity of PPARγ in Cell-Based Assays.
    J Nippon Med Sch, 85:95-101, 2018

     

    Ikarashi R, Akechi H, Kanda Y, Ahmad A, Takeuchi K, Morioka E, Sugiyama T, Ebisawa T, Ikeda M, Ikeda M: Regulation of molecular clock oscillations and phagocytic activity via muscarinic Ca2+signaling in human retinal pigment epithelial cells. Sci Rep. 7: 44175, 2017

     

    Tamaru T, Ikeda M: Circadian adaptation to cell injury stresses: a crucial interplay of BMAL1 and HSF1. J Physiol Sci, 66 (4), 303-306, 2016

     

    Okabe T, Kumagai M, Nakajima Y, Shirotake S, Kodaira K, Oyama M, Ueno M, Ikeda M: The Impact of HIF1α on the Per2 Circadian Rhythm in Renal Cancer Cell Lines. PLoS One, 9(10):e109693, 2014

     

    Ikeda M, Ikeda M: Bmal1 is an essential regulator for circadian cytosolic Ca²⁺ rhythms in suprachiasmatic nucleus neurons. J Neurosci, 34:12029- 12038, 2014

     

    Takenaka Y, Haga N, Inoue I, Nakano T, Ikeda M, Katayama S, Awata T: Identification of two nickel ion-induced genes, NCI16 and PcGST1, in Paramecium caudatum. Eukaryot Cell, 13:1181-1190, 2014

     

    Takenaka Y, Inoue I, Nakano T, Shinoda Y, Ikeda M, Awata T, Katayama S: A Novel Splicing Variant of Peroxisome Proliferator-Activated Receptor-γ (Pparγ1sv) Cooperatively Regulates Adipocyte Differentiation with Pparγ2. PloS ONE, 8(6): e65583, 2013

     

    Hida A, Kitamura S, Ohsawa Y, Enomoto M, Katayose Y, Motomura Y, Moriguchi Y, Nozaki K, Watanabe M, Aritake S, Higuchi S, Kato M, Kamei Y, Yamazaki S, Goto Y, Ikeda M, Mishima K: In vitro circadian period is associated with circadian/sleep preference. Scientific Reports, 3:2074, 2013

     

    Yang F, Inoue I, Kumagai M, Takahashi S, Nakajima Y, Ikeda M: Real-Time Analysis of the Circadian Oscillation of the Rev-Erb β Promoter. J Atheroscler Thromb, 20:267-276, 2013

     

    Noguchi T, Ikeda M, Ohmiya Y, Nakajima Y: A Dual-Color Luciferase Assay System Reveals Circadian Resetting of Cultured Fibroblasts by Co-Cultured Adrenal Glands PLoS ONE 7(5): e37093, 2012

     

    Noguchi, T, Michihata, T, Nakamura, W, Takumi, T, Shimizu, R, Yamamoto, M, Ikeda, M. Ohmiya, Y, Nakajima, Y: Dual-Color Luciferase Mouse Directly Demonstrates Coupled Expression of Two Clock Genes. Biochemistry, 49:8053-8061, 2010

     

    Takahashi S, Inoue I, Nakajima Y, Seo M, TNakano T, Kumagai M, Yang F, Komoda T, Awata T, Ikeda M, Katayama S: A promoter in the novel exon of hPPARγ directs the circadian expression of PPARγ J Atheroscler Thromb, 17:73-83, 2010

     

    Yang F, Nakajima Y, Kumagai M, Ohmiya Y, Ikeda M. The molecular mechanism regulating the autonomous circadian expression of Topoisomerase I in NIH3T3 cells. Biochem Biophys Res Commun, 380:22-27, 2009

     

    Seo M, Inoue I, Ikeda M, Nakano T, Takahashi S, Katayama S, Komoda T: Statins activate human PPARa promoter and increase PPARa mRNA expression and activation in HepG2 cells PPAR Res, 2008:316306, 2008

     

    Yoshikawa A, Shimada H, Numazawa K, Sasaki T, Ikeda M, Kawashima M, Kato N, Tokunaga K, Ebisawa T: Establishment of human cell lines showing circadian rhythms of bioluminescence. Neurosci Lett, 446:40-44, 2008

     

    Noguchi T, Ikeda M, Ohmiya Y, Nakajima Y: Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases. BMC Biotechnology, 8:40, 2008

     

    Nishide S, Honma S, Nakajima Y, Ikeda M, Baba K, Ohmiya Y, Honma K: New reporter system for Per1 and Bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues. Genes Cells, 10:1173-1182, 2006

     

    Inoue I, Shinoda Y, Ikeda M, Hayashi K, Kanazawa K, Nomura M, Matsunaga T, Xu H, Kawai S, Awata T, Komoda T, Katayama S: CLOCK/BMAL1 is involved in lipid metabolism via transactivation of the peroxisome proliferator-activated receptor (PPAR) response element. J Atheroscler Thromb, 12:169-74, 2005

     

    Nakajima Y, Kimura T, Sugata K, Enomoto T, Asakawa A, Kubota H, Ikeda M, Ohmiya Y: Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate. Biotechniques, 38:891-4, 2005

     

    Nakajima Y, Ikeda M, Kimura T, Honma S, Ohmiya Y, Honma K: Bidirectional role of orphan nuclear receptor RORalpha in clock gene transcriptions demonstrated by a novel reporter assay system. FEBS Lett, 565:122-126, 2004

     

    Iwase T, Kajimura N, Uchiyama M, Ebisawa T, Yoshimura K, Kamei Y, Shibui K, Kim K, Kudo Y, Katoh M, Watanabe T, Nakajima T, Ozeki Y, Sugishita M, Hori T, Ikeda M, Toyoshima R, Inoue Y, Yamada N, Mishima K, Nomura M, Ozaki N, Okawa M, Takahashi K, Yamauchi T: Mutation screening of the human Clock gene in circadian rhythm sleep disorders. Psychiatry Res, 09:121-128, 2002

     

    Yu W, Nomura M, Ikeda M: Interactivating feedback loops within the mammalian clock:BMAL1 is negatively autoregulated and upregulated by CRY１, CRY2 and PER2. Biochem Biophys Res Commun, 290:933-941, 2002

     

    Namihira M, Honma S, Abe H, Masubuchi S, Ikeda M, Honma K: Circadian pattern, light responsiveness and localization of rPer1 and rPer2 gene expression in the rat retina. NeuroReport, 12:0471-0475, 2001

     

    Abe H, Honma S, Namihira M, Masubuchi S, Ikeda M, Ebihara S, and Honma K: Clock gene expressions in the suprachiasmatic mucleus and other areas of the brain during thythm splitting in CS mice. Mol. Brain Res, 87:92-99, 2001

     

    Masubuchi S, Honma S, Abe H, Ishizaki K, Namihira M, Ikeda M, and Honma K: Clock genes outside the suprachiasmatic nucleus involved in manifestation of locomotor activity rhythm in rats. Eur J Neurosci, 12:4206-4214, 2000

     

    Ikeda, M, Yu, W, Hirai, M, Ebisawa, T, Honma, S, Yoshimura, K, Honma K, and Nomura M: cDNA Cloning of a Novel bHLH-PAS Transcription Factor Superfamily Gene, BMAL2; Its mRNA Expression, Subcellular Distribution, and Chromosomal Localization. Biochem Biophys Res Commun, 275:493-502, 2000

     

    Ebisawa T, Uchiyama M, Kajimura N, Kamei Y, Shibui K, Kim K, Kudo Y, Iwase T, Sugishita M, Jodoi T, Ikeda M, Ozeki Y, Watanabe T, Sekimoto M, Katoh M, Yamada N, Toyoshima R, Okawa M, Takahashi K, Yamauchi T: Genetic polymorphisms of human melatonin 1b receptor gene in circadian rhythm sleep disorders. Neurosci Lett, 280:29-32, 2000

     

    Ebisawa T, Kajimura N, Uchiyama M, Katoh M, Sekimoto M, Watanabe T, Ozeki Y, Ikeda M, Jodoi T, Sugishita M, Iwase T, Kamei Y, Kim K, Shibui K, Kudo Y, Yamada N, Toyoshima R, Okawa M, Takahashi K, and Yamauchi T: Alleic Variants of Human Melatonin 1a Receptor: Functionand Prevalence in Subjects with Circadian Rhythm Sleep Disorders. Biochem Biophys Res Commun, 262:832-837,1999

     

    Yu W, Ikeda M, Abe H, Honma S, Ebisawa T, Yamauchi T, Honma K, and Nomura M: Characterization of three splice variants and genomic organization of the mouse BMAL1 gene. Biochem Biophys Res Commun, 260:760-767,1999

     

    Namihira M, Honma S, Abe H, Tanahashi Y, Ikeda M, and Honma K: Circadian rhythms and light responsiveness of mammalian clock gene, Clock and BMAL1, transcripts in the rat retina. Neurosci Lett, 271:1-4, 1999

     

    Namihira M, Honma S, Abe H, Tanahashi Y, Ikeda M, and Honma K: Daily variation and light responsiveness of mammalian clock gene, Clock and BMAL1, transcripts in the pineal body and different areas of brain in rats. Neurosci Lett, 267:69-72, 1999

     

    Abe H, Honma S, Namihira M, Tanahashi Y, Ikeda M, Yu W, and Honma K: Phase-dependent induction by light of rat Clock gene expression in the suprachiasmatic nucleus. Mol Brain Res, 66:104-110, 1999

     

    Abe H, Honma S, Namihira M,Tanahashi Y, Ikeda M, and Honma K: Circadian rhythm and light responsiveness of BMAL1 expression, a mammalian clock gene, in the suprachiasmatic nucleus of rats. Neurosci Lett, 258:93-96, 1998

     

    Honma S, Ikeda M, Abe H, Tanahashi Y, Namihira M, Honma K, and Nomura M: Circadian oscillation of BMAL1, a partner of a mammalian clock gene Clock, in rat suprachiasmatic nucleus. Biochem Biophys Res Commun, 250:83-87, 1998

     

    Kaneko A, Narabayashi Y, Itokawa K, Nakazato Y, Hosokawa T, Iwasaki S, Ohno R, Hamaguchi K, Ikeda M, and Nomura M: A case of Machado-Joseph disease presenting with spastic paraparesis. J Neurol Neurosurg Psychiatry, 62: 542-543,1997

     

    Ikeda M and Nomura M: cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS protein (BMAL1) and identification of alternatively spliced variants with alternative translation initiation site usage. Biochem Biophys Res Commun, 233: 258-264, 1997

     

    Saitoh K, Mikuni M, Ikeda M, Yamazaki C, Tomita U, and Takahashi K: Serotonin-induced 5HT1A receptor desentitization in C6BU-1 glioma cells transfected with 5-HT1A receptor gene. Neurosci Lett, 199:191-194, 1995

     

    ・連絡先：
    mikeda(at)saitama-med.ac.jp
    ・電話： 049-276-1149（内線　2079）