4. in vivo電気穿孔法を用いた眼咽頭筋ジストロフィ発症機序の解明
稀少疾患OPMDの原因遺伝子PABPN1遺伝子の疾患変異型をマウス筋肉組織へin vivo 電気穿孔法により導入し、OPMDの疾患モデル動物を作出することによって、個別の病理を分子レベルで理解し、将来的な治療法の開発を目指す。 神経内科(荒木信夫教授、溝井令一先生）、池田教授、熊谷助手との共同研究

＜業績リスト＞

Cytoskeletal Regulation by AUTS2 in Neuronal Migration and Neuritogenesis

Cell Rep. 2014 Dec 24;9(6):2166-2179.

doi: 10.1016/j.celrep.2014.11.045. Epub 2014 Dec 18.

3)Orihara M., Toriya M., *Nakao K. and Okano H.

4)Ozawa Y, Nakao K, Kurihara T, Shimazaki T, Shimmura S, Ishida S, Yoshimura A, Tsubota K, Okano H.
Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.　
J Biological Chemistry 283:24561-24670 (2008)

5)Ozawa Y, Nakao K., Shimazaki T, Shimmura S, Kurihara T, Ishida S, Yoshimura A, Tsubota K, Okano H.
SOCS3 is required to temporally fine-tune photoreceptor cell differentiation.
Dev Biol. 303(2):591-600 (2007). Epub 2006 Dec 1.

6)Toriya M., Tokunaga A., Sawamoto K., Nakao K.* and Okano H.*(*Correspondence)
Distinct functions of human Numb isoforms revealed by misexpression in neural stem cell lineage in the Drosophila larval brain.
Dev. Neurosci. 28(1-2):142-55 (2006)　

7)Kohyama J., Tokunaga A., Fujita Y., Miyoshi H., Nagai T., Miyawaki A., Nakao K., Mastuzaki Y. and Okano H.
Visualization of spatio-temporal activation of Notch signaling: live monitoring and significance in neural development. 
Dev. Biol. 286:311-325 (2005)

8)Ozawa, Y., Nakao, K.., Shimazaki, T., Takeda, J,, Akira, S., Ishihara, K., Hirano, T., Oguchi., Y. and Okano, H.
Downregulation of STAT3 activation is requires for presumptive rod photo receptor cells to differentiate in the postnatal retina.
Molecular and Cellular Neuroscience 26(2):258-270 (2004).

9)Tokunaga, A. Kohyama, J., Nakao, K. Sawamoto, K. and Okano, H.
Mapping spatio-temporal activation of Notch signaling during neurogenesis and gliogenesis in the developing mouse brain.
J. Neurochemistry 90(1):142-154 (2004).

10)Yoshida, T., Tokunaga, A., Nakao, K and Okano, H
Distinct expression patterns of splicing isoforms of mNumb in the endocrine lineage of developing pancreas.
Differentiation 71(8):486-495 (2003).

11)Kishi N, Tang Z, Maeda Y, Hirai A, Mo R, Ito M, Suzuki S, Nakao K, Kinoshita T, Kadesch T, Hui C,Artavanis-Tsakonas S, Okano H, Matsuno K.
Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis.
Int. J. Dev. Neurosci.:19(1):21-35 (2001).

12)Castella, P., Sawai,S., Nakao, K.,Wagner, J.A. and Caudy, M.
Hes1 represses both transcription repression and proliferation in PC12 cells:
A role for the Helix III/IV domain in transcription repression
Molecular Cellular Biol. 20(16):6170-83 (2000).

13)Nakao, K and Campos-Ortega, J. A.
Persistent expression of genes of the enhancer of split complex suppresses neural development in Drosophila.
Neuron 16(2):275-86(1996).


【知的財産】

藤谷与士夫、原朱美、中尾啓子、綿田裕孝　
抗膵ポリペプチド特異的モノクローナル抗体　
特願2015-2469127.

岡野栄之、徳永暁憲、神山淳、中尾啓子
Notchシグナル伝達系活性化検知方法　
特許第4599610号 特願2004-298241
平成22年10月8日